Coding

Part:BBa_K4214004:Design

Designed by: Jol Tekoniemi   Group: iGEM22_Stockholm   (2022-10-08)


hGIF with LacI promotor, pelB sequence and 6xHis-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 280
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 557
    Illegal BsaI.rc site found at 1237
    Illegal SapI site found at 1227


Design Notes

The parts were cloned together by inserting parts BBa_K4214002 and BBa_J32015 into a vector containing lacI (BBa_J04500), using restriction cloning.


Source

GIF originates from Human genome, chromosome 11q12.1. The pelB comes from Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora), and lacI is from E. coli

References