![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4214004:Design
Designed by: Jol Tekoniemi Group: iGEM22_Stockholm (2022-10-08)
hGIF with LacI promotor, pelB sequence and 6xHis-tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 280
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 557
Illegal BsaI.rc site found at 1237
Illegal SapI site found at 1227
Design Notes
The parts were cloned together by inserting parts BBa_K4214002 and BBa_J32015 into a vector containing lacI (BBa_J04500), using restriction cloning.
Source
GIF originates from Human genome, chromosome 11q12.1. The pelB comes from Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora), and lacI is from E. coli